产品货号：
GS4565
中文名称：
重组小鼠FGF-acidic
英文名称：
Recombinant Murine Acidic Fibroblast Growth Factor
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 15.8kDa,a single non-glycosylated polypeptide chain containing 140 amino acid residues.
储存条件：冷冻（-20℃）
概述
Murine aFGF,encoded by the FGF1 gene,is a member of the fibroblast growth factor(FGF)family.Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate.After further fractionating the extract using acidic and basic pH,two different forms have isolated that named "acidic fibroblast growth factor"(FGF-1)and "basic fibroblast growth factor"(FGF-2).Murine aFGF shares 52%amino acid sequence identity with bFGF.Murine aFGF shares 96%amino acid sequence identity with human aFGF,so it exhibits considerable species crossreactivity between murine and human aFGF.In mammalian FGF receptor family has 4 members,FGFR1,FGFR2,FGFR3,and FGFR4,and 1,2,3 have 2 sub-types “b”,“c”.aFGF can bind and activate all 7 different FGFRs.Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan.aFGF plays an important role in the regulation of cell survival,cell division,angiogenesis,cell differentiation and cell migration.aFGF are also involved in a variety of biological processes,including embryonic development,morphogenesis,tissue repair,tumor growth and invasion.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组小鼠FGF-acidic，FGF-acidic, Murine
储存条件：冷冻（-20℃）
概述
Murine aFGF,encoded by the FGF1 gene,is a member of the fibroblast growth factor(FGF)family.Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate.After further fractionating the extract using acidic and basic pH,two different forms have isolated that named "acidic fibroblast growth factor"(FGF-1)and "basic fibroblast growth factor"(FGF-2).Murine aFGF shares 52%amino acid sequence identity with bFGF.Murine aFGF shares 96%amino acid sequence identity with human aFGF,so it exhibits considerable species crossreactivity between murine and human aFGF.In mammalian FGF receptor family has 4 members,FGFR1,FGFR2,FGFR3,and FGFR4,and 1,2,3 have 2 sub-types “b”,“c”.aFGF can bind and activate all 7 different FGFRs.Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan.aFGF plays an important role in the regulation of cell survival,cell division,angiogenesis,cell differentiation and cell migration.aFGF are also involved in a variety of biological processes,including embryonic development,morphogenesis,tissue repair,tumor growth and invasion.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞96%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2ng/ml,corresponding to a specific activity of>5.0×106 IU/mg in the presence of 10μg/ml of heparin. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rMuaFGF as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD |
相关搜索：重组小鼠FGF-acidic，FGF-acidic, Murine